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NovoAlign

Powerful tool designed for mapping of short reads onto a reference genome from Illumina, Ion Torrent, and 454 NGS platforms.

 request trialdownload nowdocumentation

 

Packed with unsurpassed features:

  1. Mapping with base quality values.
  2.  Alignment quality scores using posterior alignment probability.
  3. Paired end alignment.
  4. Mismatches and gaps of up to 50% of read length.
  5. Use of ambiguous codes in reference sequences can be used to reduce allelic bias.
  6. Bisulphite alignment mode (except for NovoalignCS) for analysis of methylation status.
  7. Automatic base quality calibration.
  8. Handles single end and paired end reads up to 950bp/read.
  9. In built adapter trimming and base quality trimming.
  10. Option for amplicon primer trimming.

High sensitivity allows you to map more reads than other aligners:

 

This is a generated report from Genome Comparison & Analytic Testing (GCAT), using a read length of 400 base pair, single ended, UCSC Human Genome HG19 data; with sequencing errors, SNPS and relatively high volume of indels introduced into the data.

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Note: From the table and charts above, NovoAlign3 performed better in all the aspect of alignment, with higher total correct mapped, and significantly lower incorrectly mapped reads.

(Source: http://www.bioplanet.com/gcat/reports/209/alignment/400bp-se-large-indel/novoalign3/compare-49-61/read-has-no-indels

 

mapped vs error

 

Note: An older version NovoAlign (V2) was used in this comparison test report. NovoAlign V2 has scored highest in sensitivity compared to other aligners. The test results may improve on NovoAlign V3.

(Source: http://arxiv.org/pdf/1303.3997v2.pdf)

Uncompromised accuracy, to minimize false callings of your alignment:

This is a generated report from Genome Comparison & Analytic Testing (GCAT), using a read length of 400 base pair, single ended, UCSC Human Genome HG19 data; with sequencing errors, SNPS and relatively high volume of indels introduced into the data.

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Note: This graph shows the percentage of incorrectly aligned reads (false positive rate) plotted against the percentage of correctly aligned reads (true positive rate). Reads with the highest amp quality should have the lowest frequency of incorrect reads with the frequency increasing as the map quality decreases. The report shows that NovoAlign (V3) has the edge over the other aligners when it comes to accuracy.

(Source: http://www.bioplanet.com/gcat/reports/209/alignment/400bp-se-large-indel/novoalign3/compare-49-61/read-has-no-indels

nbt.2835-F2

 

Fully backed up by extensive technical support, giving you the assurance and confidence in your research needs:

Explore, and post questions in our information rich support forum to seek the best answers to all your technical questions.

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(Source: http://novocraft.com/wiki/tiki-view_forum.php?forumId=1)

Market’s leading aligner, currently licensed by over 100 institutions, research centers, universities and private corporations.

 

Our licensees:

 


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